ASSOCIATION OF POLYMORPHISM IN OLRI GENE WITH MILK PRODUCTION TRATIS IN IRANIAN HOLSTEIN DAIRY CATTLE
1th International Biotechnology World Congress, 2012, Dubai
The role of oxidized low-density lipoprotein receptor 1 (OLR1) in lipid metabolism and the results of previous QTL studies prompted the investigation of OLR1 as a candidate gene affecting milk production traits. The present study investigated the impact of single nucleotide polymorphism ( ) in the untranslated region of OLR1 gene (OLR1 g.8232 C>A) on milk production traits in Holstein Dairy Cattle. The analysis was conducted on 408 Iranian Holstein cows in five farms located in Isfahan province. Genotypes were identified using PCR-RFLP technique. Fragments with 270 bp indicated allele A and those with 250 bp and 20 bp represented allele C. Using SAS software (Proc GLM). The effect of the polymorphism on milk production traits was investigated. Results showed that individuals with genotype CC had significantly more fat percentage in comparison with genotypes AC and AA (P<0.001). furthermore cows carrying genotype CC and AC showed significantly more milk fat yield compared to genotype AA (P<0.01). this or another that is in linkage disequilibrium, might influence the expression level of OLRI. Regarding the association between the polymorphism and these traits, the has potential to be used as a marker in marker- assister selection programs.