Evaluation of OLR1 gene polymorphism and diversity in Iranian Holstein dairy cattle

To investigate the OLR1 gene polymorphism and diversity in Iranian Holstein dairy cattle, blood samples werw obtained from 408 cows in 5 industrial dairy farms in Isfahan province. After extracting DNA, determining the quality and quantity of extracted DNA, amplification and digestion by restriction enzyme, digestion product was observed on a 2.5% agarose gel. Alleles A(uncut 270bp) and (cut into fragments 250bp and 20bp) were observed in all 5 herds. Genetic analysis was done by software PopGen32. Calculated indices showed high diversity for this polymorphism in studied population that can be used in Marker-Assisted Selection.