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Frequency of the Bovine Acyl-CoA:Diacylglycerol Acyltransferase 1 (DGAT1) K232A Polymorphism in Iranian Holstein Dairy Cattle

Because of the DGAT1 K232A polymorphism in the diversification of milk fat has been proven, This study were to assess the frequency of DGAT1 K232A polymorphism in Iranian Holstein dairy cows. Acyl-CoA:diacylglycerol acyltransferase 1 (DGAT1) , which is encoded by the DGAT1 gene,  is a key enzyme in triacylglycerol synthesis in the mammary gland. The  considered polymorphism  results  from AA→GC nucleotide  substitution in DGAT1 gene exon 8 and so, alanine to lysine amino acid change at product protein. This polymorphism proved to significantly affect the percent fat content in milk. Genotyping  population, was performed using RFLP-PCR technique. A 411 bp fragment including this polymorphism was amplified and digested with  the  enzyme  CfrI  to  determine  the  genotypes  of  408  Holstein  cows.Three  types genotype KK, KA and AA were detected. KK individuals have not mutatnt alles, KA individuals have one mutant allele and AA individual have twomutant alleles. Frequency of genetic AA, KA and  KK genotypes were respectively 0/3578, 0.5515,  and 0.0907 and frequency of  K and A allels  were estimated 0.37 and 0.63, respectively.

Keywords: DGAT1 gene, K232A polymorphism, RFLP-PCR,  Iranian Holstein Cattle

 

Conference Papers
Month/Season: 
September
Year: 
2011
File: