Influence of the bovine acyl-CoA:diacylglycerol acyltransferase 1(DGAT1)K232A genetic variation on somatic cell score and milk performance

The study aimed at estimating  the association between of the DGAT1 K232A whit somatic cell score (SCS) and milk performance Holstein cattle .The gene diacylglycerol-O-transferase 1 (DGAT1) is mapped to the centromeric end of The bovin BTA14 and encodes the acyl-CoA:diacylglycerol acyltransferase 1enzyme which catalyses the final step in triglyceride synthesis . K232A polymorphism proved to significantly affect the percent fat content in milk. Genotyping population, was performed using RFLP-PCR technique. A 411 bp fragment including was amplified and digested with  the  enzyme  CfrI  to determine  the  genotypes  of  408 Holstein cows. Three  types genotype KK, KA and AA were detected. Frequency of  K and A allels  were estimated 0.37 and 0.63, respectively. The statistical analyses showed positive and significant effects of the genotype for milk production and fat percent traits in first lactations but haven’t significant effect for scs.

Keywords: DGAT1 gene, K232A polymorphism, RFLP-PCR,  somatic cell score, Holstein Cattle.