Association of polymorphism of Leptin receptor gene with production traits of Isfahan Holstein dairy cows

The objectives of present study were to evaluate the association between Leptin receptor gene polymorphism and production traits of Holstein cows of Isfahan province. For this purpose, blood samples were collected from 395 cows of five Holstein dairy herds in Isfahan province. DNA was extracted using salting out procedure. The DNA quality and quantity were assessed by electrophoresis. The 400bp fragment of the gene was amplified by specific primers. PCR products were detected by electrophoresis on 1% TAE Agarose gel. Amplified fragment digested via T aq I restriction enzyme. Statistical analysis was done by GLM procedure of the SAS software. The observed genotypes didn't show any significant relationships with milk, fat or protein percentage.