Evaluation of relationship between the OLR1 gene with production traits in Holstein dairy cattle
To investigate the relationship between OLR1 gene and milk production traits,blood samples werw obtained from 408 cows in 5 industrial dairy farms in Isfahan province that had registered recordes. After extracting DNA, determining the quality and quantity of extracted DNA, amplification and digestion by restriction enzyme, digestion product was observed on a 2.5% agarose gel. Both alleles A(uncut 270bp) and (cut into fragments 250bp and 20bp) were observed in all 5 herds and all werw in Hardy–Weinberg equilibrium. Genotype CC compared to AA and AC showed significantly (P<0.0001) more milk fat percent and genotypes CC and AC had more milk protein percent compared to genotype AA(P<0.01).