Polymorphism of Leptin receptor gene in Holstein dairy cows of Isfahan province
The objectives of this study were to estimate the allelic and genotypic frequencies of Leptin receptor gene in Holstein cows of Isfahan Province. Blood samples were collected from 395 cows of five Holstein dairy herds in Isfahan province. DNA was extracted using salting out procedure. The quality and quantity of DNA were determined using electrophoresis. The 400 bp fragment of the gene was amplified by specific primers. PCR products were detected by electrophoresis on 1%TAE Agarose gel. Amplified fragment digested by using TaqI restriction enzyme. The genotype frequencies of BB, PB and PP were 0.11, 0.49 and 0.40, respectively. The frequencies of alleles P and B were 0.648 and 0.352, respectively. The population was in HardyWeinberg equilibrium.